Chloramphenicol-induced loss of mitochondrial DNA polymerase activity in HeLa cells

Hoppe Seylers Z Physiol Chem. 1976 Aug;357(8):1103-7. doi: 10.1515/bchm2.1976.357.2.1103.


HeLa cells exposed to chloramphenicol for approximately one cell generation were found to contain a mitochondria-associated DNA polymerase with a significantly lower specific activity than that of control cells. This observation was not due to the presence of inhibitors in mitochondrial DNA polymerase preparations of chloramphenicol-treated cell cultures. In addition, there was no accumulation of a typical mitochondrial DNA polymerase in the post-mitochondrial supernatant of drug-treated cells.

MeSH terms

  • Chloramphenicol / pharmacology*
  • Cytoplasm / drug effects
  • Cytoplasm / enzymology
  • DNA-Directed DNA Polymerase / isolation & purification
  • DNA-Directed DNA Polymerase / metabolism*
  • HeLa Cells / enzymology*
  • Mitochondria / drug effects
  • Mitochondria / enzymology*


  • Chloramphenicol
  • DNA-Directed DNA Polymerase