Abstract
The administration of corticosteroids induced apoptosis of thymocytes in vivo. Among various adhesion molecules examined, vascular cell adhesion molecule-1 (VCAM-1, CD106) was shown to be strongly expressed in these apoptotic cells. Flow cytometric analysis also showed the expression of VCAM-1 in apoptotic thymocytes. An RT-PCR study demonstrated the expression of VCAM-1 mRNA in thymocytes. Splenic lymphocytes and other lymphoid cell lines also expressed VCAM-1 during the process of apoptosis. VCAM-1 mRNA expression was also observed in RT-PCR performed on these cell lines.
MeSH terms
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Animals
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Apoptosis / drug effects
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Apoptosis / genetics*
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Base Sequence
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Cell Line
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DNA Primers / genetics
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Flow Cytometry
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Gene Expression / drug effects
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Gene Expression / radiation effects
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Hydrocortisone / pharmacology
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In Situ Nick-End Labeling
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Kinetics
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Lymphocytes / cytology*
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Lymphocytes / drug effects
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Lymphocytes / metabolism*
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Male
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Mice
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Mice, Inbred C57BL
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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Spleen / cytology
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Spleen / drug effects
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Spleen / metabolism
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T-Lymphocytes / cytology
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T-Lymphocytes / drug effects
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T-Lymphocytes / metabolism
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Vascular Cell Adhesion Molecule-1 / genetics*
Substances
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DNA Primers
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RNA, Messenger
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Vascular Cell Adhesion Molecule-1
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Hydrocortisone