Coronavirus MHV-3-induced apoptosis in macrophages

Virology. 1998 Oct 10;250(1):41-9. doi: 10.1006/viro.1998.9356.


Infection with mouse hepatitis virus strain 3 (MHV-3) results in lethal fulminant hepatic necrosis in fully susceptible BALB/c mice compared to the minimal disease observed in resistant strain A/J mice. Macrophages play a central role in the pathogenesis of MHV-3-induced hepatitis. In the present study we have shown that MHV-3 infection of macrophages induces these cells to undergo apoptosis. Three methods to detect apoptosis were applied: flow cytometry analysis of nuclear DNA content, fluorescence microscopic visualization of apoptotic cells labeled by the TUNEL assay, and gel electrophoresis to detect DNA laddering. Apoptosis in A/J and BALB/c macrophages was first detected at 8 h postinfection (p.i.) and reached a maximum by 12 h p.i. The degree of MHV-3-induced apoptosis was much greater in A/J-derived macrophages than in BALB/c-derived cells. Apoptosis was inversely correlated with the development of typical MHV cytopathology, namely syncytia formation. Infected macrophages from A/J mice did not form synctia in contrast to the extensive synctia formation observed in BALB/c-derived macrophages. In MHV-3-infected BALB/c macrophage cultures, apoptotic cells were not incorporated into syncytia. Apoptosis was also inversely correlated with the expression of MHV-3-induced fgl2 prothrombinase in macrophages. These results add the murine coronavirus MHV-3 to the list of RNA-containing viruses capable of inducing apoptosis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Apoptosis*
  • Cells, Cultured
  • DNA / analysis
  • Electrophoresis, Agar Gel
  • Female
  • Fibrinogen*
  • Flow Cytometry
  • Giant Cells
  • In Situ Nick-End Labeling
  • Macrophages, Peritoneal / cytology
  • Macrophages, Peritoneal / virology*
  • Mice
  • Mice, Inbred A
  • Mice, Inbred BALB C
  • Murine hepatitis virus / physiology*
  • RNA, Messenger / analysis
  • Thromboplastin / genetics
  • Virus Replication


  • Fgl2 protein, mouse
  • RNA, Messenger
  • Fibrinogen
  • DNA
  • Thromboplastin