Construction and characterization of E3-deleted bovine adenovirus type 3 expressing full-length and truncated form of bovine herpesvirus type 1 glycoprotein gD

Virology. 1998 Oct 10;250(1):220-9. doi: 10.1006/viro.1998.9351.


Using the homologous recombination machinery of E. coli, a 1.245-kb deletion was introduced in the E3 region of bovine adenovirus 3 (BAV3) genomic DNA cloned in a plasmid. Transfection of the restriction enzyme-excised, linear E3-deleted BAV3 genomic DNA into primary fetal bovine retina cells produced infectious virus (BAV3. E3d), suggesting that all the E3-specific open reading frames are nonessential for virus replication in vitro. Using a similar approach, we constructed replication-competent (BAV3.E3gD and BAV3. E3gDt) BAV3 recombinant expressing full-length (gD) or truncated (gDt) glycoprotein of bovine herpes virus 1. Recombinant gD and gDt proteins expressed by BAV3.E3gD and BAV3.E3gDt, respectively, were recognized by gD-specific monoclonal antibodies directed against conformational epitopes, suggesting that antigenicity of recombinant gD and gDt was similar to that of the native gD expressed in bovine herpes virus 1-infected cells. Intranasal immunization of cotton rats induced strong gD- and BAV3-specific IgA and IgG immune responses. These results suggest that replication-competent bovine adenovirus 3-based vectors have potential for the delivery of vaccine antigens to the mucosal surfaces of animals.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenovirus E3 Proteins / genetics
  • Administration, Intranasal
  • Animals
  • Antibodies, Viral / analysis
  • Antibodies, Viral / blood
  • Antigens, Viral / analysis
  • Cattle
  • Cell Line
  • Cytarabine / pharmacology
  • DNA, Recombinant
  • DNA, Viral / analysis
  • DNA, Viral / biosynthesis
  • Gene Deletion
  • Gene Expression
  • Genetic Vectors / genetics*
  • Herpesvirus 1, Bovine / genetics
  • Herpesvirus 1, Bovine / immunology*
  • Lung / immunology
  • Mastadenovirus / genetics*
  • Mastadenovirus / immunology
  • Nasal Mucosa / immunology
  • Nucleic Acid Synthesis Inhibitors / pharmacology
  • Sigmodontinae
  • Vaccination
  • Vaccines, DNA / administration & dosage
  • Vaccines, DNA / genetics*
  • Vaccines, DNA / immunology
  • Viral Proteins / genetics*
  • Viral Proteins / immunology
  • Viral Vaccines / administration & dosage
  • Viral Vaccines / genetics*
  • Viral Vaccines / immunology


  • Adenovirus E3 Proteins
  • Antibodies, Viral
  • Antigens, Viral
  • DNA, Recombinant
  • DNA, Viral
  • Nucleic Acid Synthesis Inhibitors
  • Vaccines, DNA
  • Viral Proteins
  • Viral Vaccines
  • bovine herpesvirus type-1 glycoproteins
  • Cytarabine