NRSF/REST Is Required in Vivo for Repression of Multiple Neuronal Target Genes During Embryogenesis

Nat Genet. 1998 Oct;20(2):136-42. doi: 10.1038/2431.

Abstract

The neuron-restrictive silencer factor NRSF (also known as REST and XBR) can silence transcription from neuronal promoters in non-neuronal cell lines, but its function during normal development is unknown. In mice, a targeted mutation of Rest, the gene encoding NRSF, caused derepression of neuron-specific tubulin in a subset of non-neural tissues and embryonic lethality. Mosaic inhibition of NRSF in chicken embryos, using a dominant-negative form of NRSF, also caused derepression of neuronal tubulin, as well as of several other neuronal target genes, in both non-neural tissues and central nervous system neuronal progenitors. These results indicate that NRSF is required to repress neuronal gene expression in vivo, in both extra-neural and undifferentiated neural tissue.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Chick Embryo
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / physiology*
  • Gene Expression Regulation, Developmental*
  • Mice
  • Molecular Sequence Data
  • Morphogenesis
  • Mosaicism
  • Mutation
  • Nervous System / embryology*
  • Neurons / physiology*
  • Repressor Proteins / genetics
  • Repressor Proteins / physiology*
  • Transcription Factors / genetics
  • Transcription Factors / physiology*
  • Tubulin / genetics
  • Zinc Fingers*

Substances

  • DNA-Binding Proteins
  • Repressor Proteins
  • Transcription Factors
  • Tubulin

Associated data

  • GENBANK/U13878