Transcriptional and translational downregulation of H-REV107, a class II tumour suppressor gene located on human chromosome 11q11-12

Oncogene. 1998 Sep 10;17(10):1305-12. doi: 10.1038/sj.onc.1202060.

Abstract

The H-rev107 tumour suppressor was isolated as a gene specifically expressed in rat fibroblasts resistant toward malignant transformation by the activated HRAS gene (Sers et al., 1997; Hajnal et al., 1994). Here we describe the human homologue of the rat H-rev107 gene. The predicted rat and human proteins are highly conserved exhibiting an overall amino acid identity of 83%. The H-REV107-1 gene is ubiquitously expressed with the exception of haematopoetic cells and tissues. In contrast, H-REV107-1 mRNA was found only in eight of 27 cell lines derived from mammary carcinoma, lung carcinoma, gastric carcinoma, kidney carcinoma, melanoma, neuroblastoma and other tumours. The H-REV107-1 protein was not detectable in any of these tumour cells. Loss of H-REV107-1 expression was not restricted to cultured human tumour cell lines, but also found in primary squamous cell carcinomas. Gross structural aberrations of the H-REV107-1 gene were absent in tumorigenic cell lines. Thus, the block to H-REV107-1 expression is achieved both at the level of transcription and translation. By fluorescence in situ hybridisation the human H-REV107-1 gene was localised to chromosome 11q11-12.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Blotting, Northern
  • Chromosome Mapping
  • Chromosomes, Human, Pair 11*
  • Down-Regulation
  • Gene Expression Regulation, Neoplastic
  • Genes, Tumor Suppressor*
  • Humans
  • Intracellular Signaling Peptides and Proteins
  • Molecular Sequence Data
  • Multigene Family
  • Phospholipases A2, Calcium-Independent
  • Protein Biosynthesis*
  • Proteins / genetics*
  • Rats
  • Sequence Analysis
  • Sequence Homology, Amino Acid
  • Tissue Distribution
  • Transcription, Genetic*
  • Tumor Cells, Cultured
  • Tumor Suppressor Proteins

Substances

  • Intracellular Signaling Peptides and Proteins
  • Proteins
  • Tumor Suppressor Proteins
  • PLAAT3 protein, human
  • Phospholipases A2, Calcium-Independent
  • Plaat3 protein, rat

Associated data

  • GENBANK/X76453
  • GENBANK/X92814