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Case Reports
. 1998 Oct 1;106(1):80-3.
doi: 10.1016/s0165-4608(98)00037-5.

Increased C-MYC Oncogene Copy Number Detected With Combined Modified Comparative Genomic Hybridization and FISH Analysis in a Richter Syndrome Case With Complex Karyotype

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Increased C-MYC Oncogene Copy Number Detected With Combined Modified Comparative Genomic Hybridization and FISH Analysis in a Richter Syndrome Case With Complex Karyotype

E Arranz et al. Cancer Genet Cytogenet. .

Abstract

Modified comparative genomic hybridization (mCGH) was performed in a Richter syndrome case with a complex karyotype to identify and map gains of DNA sequences with possible importance in the pathogenesis and progression of the tumor. The mCGH analysis revealed a more intense signal on part of the long arm of one pair of chromosomes belonging to group C. The G-banding study showed that the increased DNA-sequence copy number originated from the 8q22-->qter chromosomal region. This increase was confirmed by performing a fluorescence in situ hybridization analysis on tumor metaphases by first using a chromosome 8-specific library and subsequently a C-MYC probe, which revealed positive staining on six different regions located on six different chromosomes, each one bearing a single copy of the C-MYC oncogene. These results show the existence of C-MYC oncogene copy-number increases and confirm the usefulness of mCGH in the genetic analysis of malignancies.

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