Enhanced penicillin production by oligosaccharides from batch cultures of Penicillium chrysogenum in stirred-tank reactors

FEMS Microbiol Lett. 1998 Sep 1;166(1):165-70. doi: 10.1111/j.1574-6968.1998.tb13198.x.


Alginate and galactomannan-derived oligosaccharides enhanced the production of penicillin G when added to stirred tank reactor cultures of Penicillium chrysogenum. The addition of oligomannuronate and oligoguluronate blocks increased penicillin G yield by 47% and 49%, respectively. The effect of mannan oligosaccharides was found to be more pronounced with 69% higher yield than the control cultures. The maximum increase in the average specific productivity of the oligosaccharide augmented cultures was 55% after addition of mannan oligosaccharides. In addition, a difference was observed in all cases in the accumulation pattern of the intermediate of penicillin biosynthesis, delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine.

MeSH terms

  • Alginates / pharmacology
  • Bioreactors
  • Fermentation
  • Kinetics
  • Mannans / pharmacology
  • Oligopeptides / metabolism
  • Oligosaccharides / pharmacology
  • Penicillin G / metabolism*
  • Penicillins / biosynthesis*
  • Penicillium chrysogenum / drug effects
  • Penicillium chrysogenum / growth & development
  • Penicillium chrysogenum / metabolism*


  • Alginates
  • Mannans
  • Oligopeptides
  • Oligosaccharides
  • Penicillins
  • galactomannan
  • 5-(2-aminoadipyl)cysteinylvaline
  • Penicillin G