rDNA-RFLP and ITS1 Sequencing of Species of the Genus Fonsecaea, Agents of Chromoblastomycosis

Med Mycol. 1998 Aug;36(4):219-25.

Abstract

Amplicons of SSU and ITS1+2 rDNA of 13 strains of Fonsecaea pedrosoi and three strains of F. compacta were digested with seven restriction enzymes. In addition, the ITS1 region of 14 strains was sequenced. With both methods significant variation was found which, however, did not coincide with established species limits based on morphology.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Ascomycota / classification
  • Ascomycota / genetics*
  • Ascomycota / ultrastructure
  • Base Sequence
  • Chromoblastomycosis / microbiology*
  • DNA, Fungal / genetics
  • DNA, Ribosomal / genetics*
  • Humans
  • Microscopy, Electron, Scanning
  • Molecular Sequence Data
  • Phylogeny
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length*
  • Restriction Mapping
  • Sequence Alignment
  • Sequence Homology, Nucleic Acid
  • Species Specificity

Substances

  • DNA, Fungal
  • DNA, Ribosomal