Hepatitis E virus RNA detection in serum and feces specimens with the use of microspin columns

J Virol Methods. 1998 Oct;74(2):209-13. doi: 10.1016/s0166-0934(98)00049-4.

Abstract

This report describes the use of microspin columns for extraction of hepatitis E virus (HEV) RNA from stool and serum specimens for reverse transcription-polymerase chain reaction (RT-PCR) and compares this method with the glass powder method. The microspin column method was found to be 1- to 2-log more sensitive in detecting HEV RNA than the glass powder method and had better reproducibility. The microspin column method also detected HEV RNA in a larger number of specimens than the glass powder method from among a panel of serum and stool specimens. Use of this method may allow better assessment of viremia and fecal excretion in patients and experimental animals infected with HEV.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Disease Models, Animal
  • Feces / virology*
  • Hepatitis E / virology*
  • Hepatitis E virus / genetics
  • Hepatitis E virus / isolation & purification*
  • Humans
  • Macaca
  • RNA, Viral / blood
  • RNA, Viral / isolation & purification*
  • Reproducibility of Results
  • Reverse Transcriptase Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Specimen Handling / methods
  • Viremia / virology

Substances

  • RNA, Viral