Delineation of human antibody responses to culture filtrate antigens of Mycobacterium tuberculosis

J Infect Dis. 1998 Nov;178(5):1534-8. doi: 10.1086/314438.


This study was undertaken to define the antigens in culture filtrates of actively replicating Mycobacterium tuberculosis that are recognized by antibodies from tuberculosis (TB) patients. Two-dimensional Western blots were probed with sera from healthy controls and TB patients that were preabsorbed with Escherichia coli lysates to deplete cross-reactive antibodies. Antibodies from TB patients recognized 26 of the >100 culture filtrate proteins, and the repertoire changed with disease progression. Only 12 of 26 antigens, including 3 proteins implicated in colonization and invasion by mycobacteria (MPT51, MPT32, and 85C), and 9 (as yet undefined proteins) were reactive with sera from TB patients with early noncavitary or cavitary disease. Eight additional antigens, including 4 undefined proteins, were recognized only by sera from a subset of patients with advanced cavitary disease. Studies suggest that 3 of the antigens recognized by sera from patients with early TB (85C, MPT32, and a 88-kDa protein) have strong serodiagnostic potential.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antibodies, Bacterial / biosynthesis*
  • Antigens, Bacterial / immunology*
  • Blotting, Western
  • Cells, Cultured
  • Cross Reactions
  • Electrophoresis, Gel, Two-Dimensional
  • Escherichia coli
  • Humans
  • Mycobacterium tuberculosis / immunology*
  • Tuberculosis, Pulmonary / immunology


  • Antibodies, Bacterial
  • Antigens, Bacterial