Inhibition of influenza virus replication in tissue culture by 2-deoxy-2,3-dehydro-N-trifluoroacetylneuraminic acid (FANA): mechanism of action

J Gen Virol. 1976 Oct;33(1):159-63. doi: 10.1099/0022-1317-33-1-159.


The neuraminidase inhibitor 2-deoxy-2,3-dehydro-N-trifluoroacetylneuraminic acid (FANA) inhibits the mutlicycle replication of influenza viruses in tissue culture. Influenza virus grown in the presence of FANA contains neuraminic acid on its envelope which then serves as receptor for other virus particles causing extensive aggregation. Thus, FANA inhibits influenza virus replication by preventing the enzymatic removal of neuraminic acid from the virus envelope.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Line
  • Hemagglutinins, Viral / analysis
  • Hydroxides / pharmacology
  • Influenza A virus / enzymology
  • Influenza A virus / growth & development*
  • Influenza A virus / immunology
  • Mutation
  • Neuraminidase / antagonists & inhibitors*
  • Neuraminidase / biosynthesis
  • Sialic Acids / pharmacology*
  • Temperature
  • Virus Replication / drug effects*


  • Hemagglutinins, Viral
  • Hydroxides
  • Sialic Acids
  • Neuraminidase