Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 395 (6702), 567-74

Crystal Structure of a Hepatitis Delta Virus Ribozyme


Crystal Structure of a Hepatitis Delta Virus Ribozyme

A R Ferré-D'Amaré et al. Nature.


The self-cleaving ribozyme of the hepatitis delta virus (HDV) is the only catalytic RNA known to be required for the viability of a human pathogen. We obtained crystals of a 72-nucleotide, self-cleaved form of the genomic HDV ribozyme that diffract X-rays to 2.3 A resolution by engineering the RNA to bind a small, basic protein without affecting ribozyme activity. The co-crystal structure shows that the compact catalytic core comprises five helical segments connected as an intricate nested double pseudoknot. The 5'-hydroxyl leaving group resulting from the self-scission reaction is buried deep within an active-site cleft produced by juxtaposition of the helices and five strand-crossovers, and is surrounded by biochemically important backbone and base functional groups in a manner reminiscent of protein enzymes.

Comment in

Similar articles

See all similar articles

Cited by 261 articles

See all "Cited by" articles

Publication types

Associated data

LinkOut - more resources