The PLC1 Encoded Phospholipase C in the Yeast Saccharomyces Cerevisiae Is Essential for Glucose-Induced Phosphatidylinositol Turnover and Activation of Plasma Membrane H+-ATPase

Biochim Biophys Acta. 1998 Oct 21;1405(1-3):147-54. doi: 10.1016/s0167-4889(98)00099-8.

Abstract

Addition of glucose to glucose-deprived cells of the yeast Saccharomyces cerevisiae triggers rapid turnover of phosphatidylinositol, phosphatidylinositol-phosphate and phosphatidylinositol 4,5-bisphosphate. Glucose stimulation of PI turnover was measured both as an increase in the specific ratio of 32P-labeling and as an increase in the level of diacylglycerol after addition of glucose. Glucose also causes rapid activation of plasma membrane H+-ATPase. We show that in a mutant lacking the PLC1 encoded phospholipase C, both processes were strongly reduced. Compound 48/80, a known inhibitor of mammalian phospholipase C, inhibits both processes. However, activation of the plasma membrane H+-ATPase is only inhibited by concentrations of compound 48/80 that strongly inhibit phospholipid turnover. Growth was inhibited by even lower concentrations. Our data suggest that in yeast cells, glucose triggers through activation of the PLC1 gene product a signaling pathway initiated by phosphatidylinositol turnover and involved in activation of the plasma membrane H+-ATPase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Division / drug effects
  • Cell Membrane / enzymology
  • Diglycerides / biosynthesis
  • Enzyme Activation / drug effects
  • Genes, Fungal*
  • Glucose / metabolism
  • Glucose / pharmacology*
  • Hydrogen-Ion Concentration
  • Kinetics
  • Phosphatidylinositols / metabolism*
  • Proton-Translocating ATPases / metabolism*
  • Saccharomyces cerevisiae / drug effects
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism*
  • Signal Transduction
  • Type C Phospholipases / genetics*
  • Type C Phospholipases / metabolism*
  • p-Methoxy-N-methylphenethylamine / pharmacology

Substances

  • Diglycerides
  • Phosphatidylinositols
  • p-Methoxy-N-methylphenethylamine
  • Type C Phospholipases
  • Proton-Translocating ATPases
  • Glucose