The potentiating influences of insulin on pancreozymin-induced hyperpolarization and amylase release in the pancreatic acinar cell

J Physiol. 1976 Oct;261(3):505-21. doi: 10.1113/jphysiol.1976.sp011571.


1. Insulin (1 mu./ml.) potentiated the release of amylase from isolated pancreas of rats perfused with erythrocyte-containing medium and stimulated by 0-5 m-u. pancreozymin (Pz)/ml., whereas the same concentration of insulin failed to potentiate the response evoked by or 200 m-u. PZ/ml. 2. Intracellular measurement of membrane potentials from the acinar cells of the same preparations showed that insulin (1 mu./ml.) simultaneously potentiates the hyperpolarization and amylase release in response to 0-5 m-u. PZ/ml. 3. These effects of insulin on the PZ-induced responses were inhibited by ouabain (3 X 10(-5) M). 4. The results suggest that insulin, in the concentration studied, has a potentiating action on the activity of an electrogenic Na pump, which maintains the hyperpolarization and amylase release during continuous stimulation with Pz. 5. Insulin in also potentiated the Pz-induced amylase release in the rat pancreata in situ after vagotomy and ligature of the pyloric region.

MeSH terms

  • Amylases / metabolism*
  • Animals
  • Biological Transport, Active / drug effects
  • Cholecystokinin / pharmacology*
  • Drug Synergism
  • In Vitro Techniques
  • Insulin / pharmacology*
  • Male
  • Membrane Potentials / drug effects*
  • Ouabain / pharmacology
  • Pancreas / drug effects*
  • Pancreas / metabolism
  • Rats
  • Tachyphylaxis


  • Insulin
  • Ouabain
  • Cholecystokinin
  • Amylases