Cats are protected against feline immunodeficiency virus infection following vaccination with a homologous AP-1 binding site-deleted mutant

Arch Virol. 1998;143(9):1839-45. doi: 10.1007/s007050050422.

Abstract

Following establishment, via the vaginal route, of infection with an AP-1 binding-site deleted mutant (delta AP-1) of feline immunodeficiency virus (FIV), cats were challenged with a homologous intact strain (TM2) of FIV. The cats were observed for 23 weeks to evaluate the efficacy of the delta AP-1 against the homologous TM2 strain challenge. These two viruses were differentiated by Southern blotting after amplification of proviral DNA by semi-nested polymerase chain reaction in DNAs of peripheral blood mononuclear cells and tissues. A TM2-specific band was detected in one cat exposed to but not infected with delta AP-1, but not in two delta AP-1-infected. These results indicate that delta AP-1 could protect against subsequent challenge with homologous FIV TM2 strain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Binding Sites
  • Cats
  • DNA, Viral / analysis
  • Feline Acquired Immunodeficiency Syndrome / prevention & control*
  • Female
  • Mutation
  • Polymerase Chain Reaction
  • Proviruses / genetics
  • Transcription Factor AP-1 / immunology*
  • Vaccination
  • Vaginal Diseases / prevention & control*
  • Viral Vaccines / immunology*

Substances

  • DNA, Viral
  • Transcription Factor AP-1
  • Viral Vaccines