PTEN/MMAC1 is infrequently mutated in pT2 and pT3 carcinomas of the prostate

Oncogene. 1998 Oct 15;17(15):1979-82. doi: 10.1038/sj.onc.1202119.


Deletion of the q23-24 region of human chromosome 10 is one of the most frequent genetic alterations in prostate cancer, suggesting that inactivation of a tumor suppressor gene in this region is involved in the development or progression of this carcinoma. A candidate gene, PTEN/MMAC1, has been identified from this chromosomal region; mutations of this gene have been found in various advanced tumors and cell lines including those of prostate cancer. To further define the role of PTEN/MMAC1 in the development of prostate cancer and its spectrum of genetic alterations, we analysed 40 pT2 or pT3 prostate tumors for allelic loss, mutations, and homozygous deletions using PCR-based methods. Six tumors showed loss of heterozygosity for one of the ten markers analysed, while one tumor showed loss of two markers. None of the markers within PTEN/MMAC1 was lost. Direct sequencing of PCR amplified exons and intron/exon junctions of all 40 tumors revealed three sequence variants, one of which was a point mutation in exon 9, while the other two were polymorphisms. Using multiplex PCR, no homozygous deletions were detected in any of the neoplasms. Our results showing a low frequency of alterations of PTEN/MMAC1 in pT2 and pT3 prostate cancers suggest that this gene plays an insignificant role in the development of most low stage carcinomas of the prostate.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Base Sequence
  • DNA Primers
  • Genes, Tumor Suppressor*
  • Homozygote
  • Humans
  • Loss of Heterozygosity
  • Male
  • Middle Aged
  • Mutation*
  • PTEN Phosphohydrolase
  • Phosphoric Monoester Hydrolases*
  • Prostatic Neoplasms / genetics*
  • Prostatic Neoplasms / pathology
  • Protein Tyrosine Phosphatases / genetics*
  • Sequence Deletion
  • Tumor Suppressor Proteins*


  • DNA Primers
  • Tumor Suppressor Proteins
  • Phosphoric Monoester Hydrolases
  • Protein Tyrosine Phosphatases
  • PTEN Phosphohydrolase
  • PTEN protein, human