Molecular cloning and expression of rhesus macaque and sooty mangabey interleukin 16: biologic activity and effect on simian immunodeficiency virus infection and/or replication

AIDS Res Hum Retroviruses. 1998 Oct 10;14(15):1323-8. doi: 10.1089/aid.1998.14.1323.


Interleukin 16 (IL-16) has been shown to diminish HIV and SIV replication through inhibition of HIV and SIV mRNA transcription. To evaluate its role further, we compared IL-16 cloned from disease-susceptible rhesus macaques and disease-resistant sooty mangabeys. Recombinant rhesus macaque (rr) IL-16 was compared with recombinant sooty mangabey (rm), human, and other nonhuman primate IL-16 sequences and evaluated for its ability to induce chemotaxis and inhibit the mixed lymphocyte response (MLR). Also, rrIL-16 and rmIL-16 were evaluated for suppression of SIVmac251, which replicates efficiently in T cells and monocyte/macrophages (dual tropic), and cloned SIVmac239, which replicates efficiently in T cells (T tropic). Sequence comparison of rrIL-16 and rmIL-16 with human IL-16 showed >97% amino acid identity. Biocharacterization of rrIL-16 revealed potent induction of chemotaxis (p < 0.05) and marked inhibition of MLR (73 +/- 0.6%,p < 0.05) in rhesus and human cell systems. Using rrIL-16 and rmIL-16, p27 antigen production from PBMCs infected with SIVmac251 was decreased up to 70% (p < 0.05 and p < 0.01, respectively). In similar cultures infected with SIVmac239, rrIL-16 and rmIL-16 reduced p27 levels by 96 and 100%, respectively. These data demonstrate the biologic and antiviral functionality of rrIL-16 and rmIL-16.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cells, Cultured
  • Cercocebus atys
  • Chemotaxis, Leukocyte / drug effects
  • Cloning, Molecular
  • Humans
  • Interleukin-16 / genetics*
  • Interleukin-16 / pharmacology
  • Lymphocyte Culture Test, Mixed
  • Macaca mulatta
  • Macaca nemestrina
  • Molecular Sequence Data
  • Recombinant Proteins / genetics
  • Recombinant Proteins / pharmacology
  • Sequence Homology, Amino Acid
  • Simian Acquired Immunodeficiency Syndrome / drug therapy*
  • Simian Immunodeficiency Virus / drug effects*
  • T-Lymphocytes / cytology
  • T-Lymphocytes / drug effects
  • Virus Replication / drug effects


  • Interleukin-16
  • Recombinant Proteins