Suppression of auxin signal transduction by a MAPK cascade in higher plants

Nature. 1998 Oct 15;395(6703):716-20. doi: 10.1038/27240.

Abstract

The plant hormone auxin activates many early response genes that are thought to be responsible for diverse aspects of plant growth and development. It has been proposed that auxin signal transduction is mediated by a conserved signalling cascade consisting of three protein kinases: the mitogen-activated protein kinase (MAPK), MAPK kinase (MAPKK) and MAPKK kinase (MAPKKK). Here we show that a specific plant MAPKKK, NPK1, activates a MAPK cascade that leads to the suppression of early auxin response gene transcription. A mutation in the kinase domain abolishes NPK1 activity, and the presence of the carboxy-terminal domain diminishes the kinase activity. Moreover, the effects of NPK1 on the activation of a MAPK and the repression of early auxin response gene transcription are specifically eliminated by a MAPK phosphatase. Transgenic tobacco plants overexpressing the NPK1 kinase domain produced seeds defective in embryo and endosperm development. These results suggest that auxin sensitivity may be balanced by antagonistic signalling pathways that use a distinct MAPK cascade in higher plants.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Arabidopsis
  • Calcium-Calmodulin-Dependent Protein Kinases / metabolism*
  • Gene Expression Regulation, Plant
  • Indoleacetic Acids / antagonists & inhibitors
  • Indoleacetic Acids / pharmacology*
  • MAP Kinase Kinase Kinases
  • Phosphorylation
  • Plants / enzymology
  • Plants / metabolism*
  • Plants, Genetically Modified
  • Plants, Toxic
  • Promoter Regions, Genetic
  • Protein Tyrosine Phosphatases / metabolism
  • Protein-Serine-Threonine Kinases / genetics
  • Protein-Serine-Threonine Kinases / metabolism*
  • Protoplasts
  • Signal Transduction*
  • Tobacco
  • Zea mays

Substances

  • Indoleacetic Acids
  • Protein-Serine-Threonine Kinases
  • Calcium-Calmodulin-Dependent Protein Kinases
  • MAP Kinase Kinase Kinases
  • Protein Tyrosine Phosphatases