Human transformer-2-beta gene (SFRS10): complete nucleotide sequence, chromosomal localization, and generation of a tissue-specific isoform

Genomics. 1998 Oct 15;53(2):191-202. doi: 10.1006/geno.1998.5471.

Abstract

Htra2-beta is a human homologue of Drosophila transformer-2 and a member of the SR-like protein family. Here we report the isolation and characterization of the complete htra2-beta gene (HGMW-approved symbol SFRS10). The gene spans 21,232 bp and is composed of 10 exons and 9 introns. Radiation hybrid mapping localized the gene to chromosome 3q. The region upstream of the transcription initiation codon contains an Alu element and several potential transcription factor binding sites. RT-PCR and comparison with EST clones revealed five different RNA isoforms generated by alternative splicing. These isoforms encode three diverging open reading frames, and two of these, htra2-beta3 and htra2-beta4, lack the first SR domain. Htra2-beta3 is developmentally regulated and expressed predominantly in brain, liver testis, and weakly in kidney. Furthermore, the domain structure of htra2-beta3 resembles a variant found in the Drosophila male germline, indicating a remarkable conservation of alternative transformer-2 variants. Finally, we show that htra2-beta3 is expressed in the nucleus and interacts with a subset of SR proteins in a yeast two-hybrid system and in vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alternative Splicing
  • Animals
  • Base Sequence
  • Chromosome Mapping
  • Chromosomes, Human, Pair 3*
  • Drosophila Proteins*
  • Gene Expression Regulation
  • Humans
  • Male
  • Molecular Sequence Data
  • Nerve Tissue Proteins*
  • Nuclear Proteins / genetics*
  • Nuclear Proteins / metabolism*
  • Organ Specificity
  • Promoter Regions, Genetic
  • RNA-Binding Proteins*
  • Rabbits
  • Ribonucleoproteins / genetics*
  • Ribonucleoproteins / immunology
  • Ribonucleoproteins / metabolism*
  • Serine-Arginine Splicing Factors
  • Tissue Distribution

Substances

  • Drosophila Proteins
  • Nerve Tissue Proteins
  • Nuclear Proteins
  • RNA-Binding Proteins
  • Ribonucleoproteins
  • TRA2B protein, human
  • tra2 protein, Drosophila
  • Serine-Arginine Splicing Factors

Associated data

  • GENBANK/AF057159