Platelet-derived growth factor (PDGF)-BB stimulates osteoclastic bone resorption directly: the role of receptor beta

Biochem Biophys Res Commun. 1998 Oct 9;251(1):190-4. doi: 10.1006/bbrc.1998.9412.


The growth-promoting activity of PDGF-BB was studied on the adult osteoclasts in the present study. The PDGF receptor beta was detected on the osteoclast membrane through immunohistochemistry (LSAB method) and immunomicroscopy. The PDGF-BB was exerted on the osteoclasts that adhered to the bone slice at concentrations of 0, 10, 20, 30, and 40 ng/ml. The volume of Howship's lacuna augmented significantly and the number of resorption pits also increased with its dose (p < 0.01). The activity of both total acid phosphatase (ACP) and tartrate-resistant acid phosphatase (TRAP) increased significantly. These results suggest that PDGF-BB promotes adult osteoclastic bone resorption directly through PDGF receptor beta and is believed to play important roles in the bone healing process and reconstruction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acid Phosphatase / metabolism
  • Adult
  • Animals
  • Becaplermin
  • Bone Resorption / chemically induced
  • Bone Resorption / metabolism*
  • Bone Resorption / pathology
  • Bone and Bones
  • Cells, Cultured
  • Humans
  • Immunohistochemistry
  • Mice
  • Microscopy, Immunoelectron
  • Osteoclasts / enzymology
  • Osteoclasts / metabolism*
  • Osteoclasts / ultrastructure
  • Platelet-Derived Growth Factor / metabolism
  • Platelet-Derived Growth Factor / physiology*
  • Proto-Oncogene Proteins c-sis
  • Receptor, Platelet-Derived Growth Factor beta
  • Receptors, Platelet-Derived Growth Factor / physiology*


  • Platelet-Derived Growth Factor
  • Proto-Oncogene Proteins c-sis
  • Becaplermin
  • Receptor, Platelet-Derived Growth Factor beta
  • Receptors, Platelet-Derived Growth Factor
  • Acid Phosphatase