Establishment of the enzyme-linked immunosorbent assay for connective tissue growth factor (CTGF) and its detection in the sera of biliary atresia

Biochem Biophys Res Commun. 1998 Oct 29;251(3):748-52. doi: 10.1006/bbrc.1998.9543.


Connective tissue growth factor (CTGF) is a mitogenic, chemotactic, and cell matrix-inducing factor for fibroblasts. We generated murine monoclonal antibodies against CTGF and established a sandwich enzyme-linked immunosorbent assay (ELISA) for detection of CTGF. By using the ELISA, we confirmed that CTGF was specifically induced in human fibroblasts by TGF-beta but not by PDGF, FGF, IGF-I, or EGF. We also found that the serum levels of CTGF were significantly correlated with the progression of hepatic fibrosis in biliary atresia. These results indicated that CTGF is potentially a useful parameter for monitoring certain types of fibrotic disorders.

MeSH terms

  • Biliary Atresia / blood*
  • Biliary Atresia / complications
  • Connective Tissue Growth Factor
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Fibroblasts / drug effects
  • Growth Substances / blood*
  • Growth Substances / pharmacology
  • Humans
  • Immediate-Early Proteins*
  • Intercellular Signaling Peptides and Proteins*
  • Liver Cirrhosis / complications
  • Liver Function Tests
  • Single-Blind Method
  • Transforming Growth Factor alpha / biosynthesis


  • CCN2 protein, human
  • Growth Substances
  • Immediate-Early Proteins
  • Intercellular Signaling Peptides and Proteins
  • Transforming Growth Factor alpha
  • Connective Tissue Growth Factor