Molecular Cloning and Expression of Rat Contraception Associated Protein 1 (CAP1), a Protein Putatively Involved in Fertilization

Biochem Biophys Res Commun. 1998 Oct 20;251(2):545-9. doi: 10.1006/bbrc.1998.9512.

Abstract

Epididymal fluid from rats rendered infertile by oral administration of ornidazole contains a protein CAP1 (contraception-associated protein 1) that is absent from epididymal fluid, but present on epididymal sperm, from fertile vehicle-treated rats. The gene for CAP1 has been isolated from a rat testis cDNA library and its expression investigated in different tissues. The deduced protein sequence of CAP1 contains 189 amino-acid residues and database searches revealed a high degree of homology (83-95%) with human and mouse DJ-1 at the nucleotide and amino acid levels. Northern blot hybridisation from different rat tissues indicated that CAP1 is encoded by a 1.6-kilobase RNA transcript and seems to be ubiquitiously expressed in the rat with a high level of expression in the testis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary
  • Epididymis / physiology*
  • Fertility
  • Fertilization*
  • Gene Library
  • Humans
  • Infertility, Male / chemically induced
  • Infertility, Male / metabolism
  • Intracellular Signaling Peptides and Proteins
  • Male
  • Mice
  • Molecular Sequence Data
  • Oncogene Proteins / chemistry
  • Organ Specificity
  • Ornidazole / pharmacology
  • Protein Biosynthesis
  • Protein Deglycase DJ-1
  • Proteins / chemistry
  • Proteins / genetics*
  • Rats
  • Rats, Sprague-Dawley
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / chemistry
  • Sequence Alignment
  • Spermatozoa / metabolism*

Substances

  • DNA, Complementary
  • Intracellular Signaling Peptides and Proteins
  • Oncogene Proteins
  • Proteins
  • Recombinant Proteins
  • contraception associated protein 1
  • Ornidazole
  • PARK7 protein, human
  • Protein Deglycase DJ-1

Associated data

  • GENBANK/AJ007291