Effect of 26,26,26,27,27,27-Hexafluoro-1,25-dihydroxyvitamin D3 on the expression of vitamin-D-responsive genes in vitamin-D-deficient mice

Pharmacology. 1998 Dec;57(6):286-94. doi: 10.1159/000028254.

Abstract

26,26,26,27,27,27-Hexafluoro-1,25-dihydroxyvitamin D3 (ST-630) is a newly developed agent to maintain the levels of calcium and phosphorus in blood. Herein, we investigated the effect of this compound on the expression of vitamin-D-responsive genes in vitamin-D-deficient mice. ST-630 was more effective than 1, 25-dihydroxyvitamin D3 [1,25(OH)2D3] with respect to the induction of Cyp24 and calbindin-D9k mRNAs in the kidney and in the small intestine. Moreover, the increase in mRNA levels of vitamin-D-responsive genes induced by ST-630 lasted longer than that induced by 1,25(OH)2D3. These results indicate that ST-630 was more effective in inducing Cyp24 and calbindin-D9k gene expression than 1, 25(OH)2D3 when both compounds were injected into vitamin-D-deficient mice.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calbindins
  • Calcitriol / analogs & derivatives*
  • Calcitriol / pharmacology
  • Calcitriol / therapeutic use*
  • Calcium / blood
  • Cytochrome P-450 Enzyme System / biosynthesis
  • Enzyme Induction / drug effects
  • Gene Expression Regulation / drug effects
  • Injections, Intravenous
  • Intestine, Small / drug effects*
  • Intestine, Small / enzymology
  • Kidney / drug effects*
  • Kidney / metabolism
  • Male
  • Mice
  • Mice, Inbred ICR
  • RNA, Messenger / genetics
  • S100 Calcium Binding Protein G / drug effects
  • S100 Calcium Binding Protein G / genetics
  • Steroid Hydroxylases / biosynthesis
  • Vitamin D / genetics*
  • Vitamin D / metabolism
  • Vitamin D Deficiency / blood
  • Vitamin D Deficiency / drug therapy*
  • Vitamin D3 24-Hydroxylase

Substances

  • Calbindins
  • RNA, Messenger
  • S100 Calcium Binding Protein G
  • S100g protein, mouse
  • Vitamin D
  • Cytochrome P-450 Enzyme System
  • Steroid Hydroxylases
  • Vitamin D3 24-Hydroxylase
  • Calcitriol
  • 26,26,26,27,27,27-hexafluoro-1,25-dihydroxyvitamin D3
  • Calcium