Stromal progesterone receptors mediate the inhibitory effects of progesterone on estrogen-induced uterine epithelial cell deoxyribonucleic acid synthesis

Endocrinology. 1998 Nov;139(11):4708-13. doi: 10.1210/endo.139.11.6317.


The role of epithelial and stromal progesterone (P) receptors (PR) in the regulation of uterine epithelial DNA synthesis by P was investigated by analyzing the four types of tissue recombinants prepared with uterine stroma (S) and epithelium (E) from wild-type (wt) and PR knockout (PRKO) mice: wt-S + wt-E, PRKO-S + PRKO-E, wt-S + PRKO-E, and PRKO-S + wt-E. 17-Beta estradiol (E2) stimulated DNA synthesis in all four types of tissue recombinants. On the other hand, P inhibited E2-induced DNA synthesis only in tissue recombinants prepared with wild-type (PR-positive) stroma (wt-S + wt-E or wt-S + PRKO-E) but not knockout (PR-negative) stroma (PRKO-S + wt-E or PRKO-S + PRKO-E). These results clearly demonstrate that the inhibitory effect of P on uterine epithelial DNA synthesis is mediated by stromal PR. Epithelial PR is neither necessary nor sufficient for P inhibition of E2-induced epithelial DNA synthesis.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Autoradiography
  • DNA / biosynthesis*
  • Epithelial Cells / drug effects
  • Epithelial Cells / metabolism*
  • Estradiol / biosynthesis
  • Estradiol / pharmacology
  • Estrogens / pharmacology*
  • Female
  • Immunohistochemistry
  • Mice
  • Mice, Inbred BALB C
  • Progesterone / pharmacology*
  • Receptors, Progesterone / metabolism*
  • Recombination, Genetic
  • Stromal Cells / metabolism*
  • Uterus / drug effects
  • Uterus / metabolism*


  • Estrogens
  • Receptors, Progesterone
  • Progesterone
  • Estradiol
  • DNA