Use of a recombinant maedi-visna virus protein ELISA for the serologic diagnosis of lentivirus infections in small ruminants

Can J Vet Res. 1998 Oct;62(4):307-10.

Abstract

Highly purified recombinant gag and env proteins derived from Icelandic strain 1514 of maedi-visna virus were used in an indirect enzyme immunoassay (ELISA) to detect antibodies to small ruminant lentiviruses in sheep and goat sera. The recombinant protein-based ELISA performed very well relative to whole maedi-visna virus and whole caprine arthritis-encephalitis-virus-based ELISAs in its ability to detect anti-maedi visna virus and anti-caprine arthritis-encephalitis virus antibodies, despite the antigenic and genomic variability that is known to exist within and between these two small ruminant lentiviruses. The data suggest that these recombinant maedi-visna virus proteins can be reliably used in an ELISA for the routine serodiagnosis of lentiviral infections in sheep and goats.

MeSH terms

  • Animals
  • Antibodies, Viral / analysis
  • Enzyme-Linked Immunosorbent Assay
  • Goat Diseases / diagnosis*
  • Goat Diseases / virology
  • Goats
  • Pneumonia, Progressive Interstitial, of Sheep / diagnosis*
  • Pneumonia, Progressive Interstitial, of Sheep / virology
  • Recombinant Proteins / analysis
  • Serologic Tests / methods
  • Serologic Tests / veterinary
  • Sheep
  • Sheep Diseases / diagnosis*
  • Sheep Diseases / virology
  • Viral Proteins / analysis*
  • Visna / diagnosis*
  • Visna / virology
  • Visna-maedi virus / genetics*

Substances

  • Antibodies, Viral
  • Recombinant Proteins
  • Viral Proteins