Perforated patch recording with beta-escin

Pflugers Arch. 1998 Nov;436(6):1021-3. doi: 10.1007/pl00008086.

Abstract

Perforated patch recording with nystatin, amphotericin B and gramicidin can be more difficult in the hands of some investigators than others. In addition, it is difficult to introduce low molecular weight substances such as dyes into the cytoplasm in such experiments. We have determined that beta-escin represents a convenient, easy-to-use alternative to less water-soluble ionophores.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amphotericin B
  • Animals
  • Calcium Channels / physiology
  • Electric Conductivity
  • Electric Impedance
  • Escin*
  • Fluorescent Dyes
  • Gramicidin
  • Heart / physiology*
  • Kinetics
  • Male
  • Nystatin
  • Patch-Clamp Techniques*
  • Rats
  • Rats, Sprague-Dawley

Substances

  • Calcium Channels
  • Fluorescent Dyes
  • Nystatin
  • Gramicidin
  • Escin
  • Amphotericin B