Cloning of STK13, a Third Human Protein Kinase Related to Drosophila Aurora and Budding Yeast Ipl1 That Maps on Chromosome 19q13.3-ter

Genomics. 1998 Nov 1;53(3):406-9. doi: 10.1006/geno.1998.5522.

Abstract

This report describes the identification of a cDNA encoding STK13, a third human protein kinase related to the Drosophila Aurora and the budding yeast Ipl1 kinases. After screening of a human placental cDNA library with a Xenopus laevis cDNA encoding the pEg2 protein kinase and 5' RACE on testis mRNA, a full-length cDNA was isolated. The chromosomal localization of STK13 on 19q13.3-ter between the markers D19S210 and D19S218 was established by a combination of somatic cell and radiation hybrid panel PCR screening. The localization of STK13 on human chromosome 19 was confirmed by fluorescence in situ hybridization (FISH) using a genomic clone containing STK13 as a probe.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Aurora Kinase C
  • Aurora Kinases
  • Base Sequence
  • Chromosome Mapping
  • Chromosomes, Human, Pair 19 / genetics*
  • Cloning, Molecular
  • DNA Primers / genetics
  • DNA, Complementary / genetics
  • DNA, Complementary / isolation & purification
  • Drosophila / genetics
  • Female
  • Humans
  • In Situ Hybridization, Fluorescence
  • Intracellular Signaling Peptides and Proteins
  • Male
  • Molecular Sequence Data
  • Pregnancy
  • Protein Kinases / genetics*
  • Protein-Serine-Threonine Kinases / genetics*
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae Proteins*
  • Xenopus laevis / genetics

Substances

  • DNA Primers
  • DNA, Complementary
  • Intracellular Signaling Peptides and Proteins
  • Saccharomyces cerevisiae Proteins
  • Protein Kinases
  • AURKC protein, human
  • Aurora Kinase C
  • Aurora Kinases
  • IPL1 protein, S cerevisiae
  • Protein-Serine-Threonine Kinases

Associated data

  • GDB/9837399
  • GENBANK/AF059681
  • GENBANK/AF059682