Abstract
The bifunctional autolysin gene (atl) of Staphylococcus aureus was transcribed into a 4.1-kb transcript. The transcription initiation site was located at an adenine residue 33-nt upstream from the putative atl start codon. Analysis using a promoter-reporter plasmid showed that promoter activity increased during the exponential growth phase. The Tn551 insertion site of the autolysis-deficient mutant S. aureus RUSAL2 was located in the putative catalytic region of the glucosaminidase domain.
MeSH terms
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Base Sequence
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Blotting, Northern
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DNA Transposable Elements
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Genes, Bacterial / genetics*
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Genes, Reporter
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Hexosaminidases / genetics
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Molecular Sequence Data
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Mutation
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N-Acetylmuramoyl-L-alanine Amidase / genetics*
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Polymerase Chain Reaction
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Promoter Regions, Genetic
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RNA, Messenger / genetics
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Staphylococcus aureus / enzymology
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Staphylococcus aureus / genetics*
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Staphylococcus aureus / growth & development
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Transcription, Genetic
Substances
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DNA Transposable Elements
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RNA, Messenger
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Hexosaminidases
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N-Acetylmuramoyl-L-alanine Amidase