Characterization of genes for two-component phosphorelay mediators with a single HPt domain in Arabidopsis thaliana

FEBS Lett. 1998 Oct 16;437(1-2):11-4. doi: 10.1016/s0014-5793(98)01188-0.

Abstract

Three cDNAs that encode two-component phosphorelay-mediator-like proteins were cloned from Arabidopsis thaliana. Putative proteins (ATHP1-3) contain an HPt (Histidine-containing Phospho transfer)-like domain with a conserved histidine and some invariant residues that are involved in phosphorelay. Growth retardation of YPD1-disrupted yeast cells was reversed with ATHPs, which indicates that ATHPs function as phosphorelay mediators in yeast cells. The ATHP genes are expressed more in roots than in other tissues, similar to the expression of genes for a sensor histidine kinase, ATHK1, and response regulators ATRR1-4. These results suggest that ATHPs function as two-component phosphorelay mediators between sensor histidine kinase and response regulators in Arabidopsis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / genetics*
  • Arabidopsis Proteins*
  • Cloning, Molecular
  • Conserved Sequence
  • Molecular Sequence Data
  • Phosphorylation
  • Plant Proteins / genetics*
  • Proteins / genetics*
  • Sequence Alignment

Substances

  • ATHP proteins, Arabidopsis
  • Arabidopsis Proteins
  • Plant Proteins
  • Proteins

Associated data

  • GENBANK/AB012568
  • GENBANK/AB012569
  • GENBANK/AB012570