UV-C Radiation Induces Apoptotic-Like Changes in Arabidopsis Thaliana

FEBS Lett. 1998 Oct 16;437(1-2):131-6. doi: 10.1016/s0014-5793(98)01208-3.

Abstract

With a view to studying programmed cell death in plants at the molecular level, we report here for the first time that apoptotic-like changes are induced by UV radiation in plant nuclei. In Arabidopsis thaliana seedlings a UV-C dose of 10-50 kJ/m2 induces an oligonucleosomal DNA fragmentation which is reminiscent of the apoptotic DNA ladder described in animal cells. This DNA fragmentation was also detected in situ in protoplast nuclei as soon as 2 h after UV-C treatment. Moreover, UV-C induced a nuclear morphology characteristic of animal apoptotic nuclei. We propose that UV-C induction constitutes a powerful tool to compare the cellular response to irreversible UV damage in plants to that in animals and to study programmed cell death in A. thaliana.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis*
  • Arabidopsis / radiation effects*
  • Arabidopsis / ultrastructure
  • Blotting, Southern
  • DNA Fragmentation
  • Protoplasts / radiation effects
  • Seeds / radiation effects
  • Time Factors
  • Ultraviolet Rays*