The expression of the Leishmania infantum KMP-11 protein is developmentally regulated and stage specific

Biochim Biophys Acta. 1998 Nov 8;1442(2-3):230-7. doi: 10.1016/s0167-4781(98)00176-6.

Abstract

Transcription of the gene coding for the KMP-11 protein of Leishmania infantum results in the production of a mature RNA transcript of 1.3 kb in length. The expression of KMP-11 mRNA is strongly down-regulated not only during the parasite growth from the logarithmic to the stationary phase but also during the differentiation transit from promastigotes to amastigotes. The estimated concentration of KMP-11 is one order of magnitude higher in promastigotes than in amastigotes. The analysis of the Triton X-114 phase partition of the protein shows that, in agreement with its predicted secondary structure, KMP-11 has an amphipathic nature since it is found in the aqueous as well as in the detergent phase. By fluorescence microscopy a defined pattern of distribution of the protein was observed only in promastigotes where KMP-11 is mainly located in the flagellum and the flagellar pocket.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cricetinae
  • Dog Diseases / parasitology
  • Dogs
  • Enzyme-Linked Immunosorbent Assay
  • Gene Expression Regulation, Developmental*
  • Leishmania infantum / genetics*
  • Leishmania infantum / growth & development
  • Leishmania infantum / isolation & purification
  • Leishmaniasis, Visceral / parasitology
  • Leishmaniasis, Visceral / veterinary
  • Membrane Glycoproteins / biosynthesis
  • Membrane Glycoproteins / genetics*
  • Mesocricetus
  • Protozoan Proteins / biosynthesis
  • Protozoan Proteins / genetics*
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • RNA, Protozoan / biosynthesis
  • RNA, Protozoan / genetics
  • Spleen / parasitology
  • Transcription, Genetic

Substances

  • Membrane Glycoproteins
  • Protozoan Proteins
  • RNA, Messenger
  • RNA, Protozoan
  • kinetoplastid membrane protein 11, Leishmania donovani