cDNA cloning, gene characterization and 13q14.3 chromosomal assignment of CHC1-L, a chromosome condensation regulator-like guanine nucleotide exchange factor

Genomics. 1998 Nov 15;54(1):99-106. doi: 10.1006/geno.1998.5498.

Abstract

We report the characterization of a new gene mapped at chromosome band 13q14.3 telomeric to the retinoblastoma gene. This gene, designated CHC1L (for chromosome condensation 1-like), is composed of 14 exons spanning 30 kb of genomic DNA and encodes a ubiquitously expressed 3-kb mRNA. The N-terminal half of the deduced amino acid sequence shows strong homology with the seven tandem repeat structure of the regulator of chromosome condensation RCC1, which acts as a guanine nucleotide exchange factor (GEF) protein for the Ras-related GTPase Ran. CHC1L appears to be a new member of the RCC1-related GEF family.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Chromosome Mapping
  • Chromosomes, Human, Pair 13 / genetics
  • Cloning, Molecular*
  • DNA, Complementary
  • Guanine Nucleotide Exchange Factors
  • Humans
  • Molecular Sequence Data
  • Proteins / chemistry
  • Proteins / genetics*
  • Proteins / metabolism
  • RNA, Messenger / analysis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Alignment
  • Tumor Cells, Cultured
  • ras Guanine Nucleotide Exchange Factors

Substances

  • DNA, Complementary
  • Guanine Nucleotide Exchange Factors
  • Proteins
  • RNA, Messenger
  • ras Guanine Nucleotide Exchange Factors

Associated data

  • GENBANK/AF060219