An Arabidopsis immunophilin, AtFKBP12, binds to AtFIP37 (FKBP interacting protein) in an interaction that is disrupted by FK506

Plant J. 1998 Sep;15(6):783-9. doi: 10.1046/j.1365-313x.1998.00248.x.


AtFKBP12 is an Arabidopsis cDNA that encodes a protein similar to the mammalian immunophilin, FKBP12. AtFKBP12 was used as 'bait' in a yeast 2-hybrid system to screen for cDNAs in Arabidopsis encoding proteins that bind to FKBP12. Two partial cDNAs were recovered encoding the C-terminus of a protein we have called Arabidopsis thaliana FKBP12 interacting protein 37 (AtFIP37). AtFIP37 is similar to a mammalian protein, FAP48, that also binds to FKBP12. The interaction between AtFKBP12 and AtFIP37 in the 2-hybrid system, as assessed by histidine auxotrophy and beta-galactosidase activity, was disrupted by FK506, but not by cyclosporin A, a drug that binds to cyclophilin A. AtFIP37 was also shown to bind in vitro to AtFKBP12 in GST-fusion protein binding assays. The binding was abolished by prior incubation of AtFKBP12 with FK506. These findings indicate that an Arabidopsis FKBP12 ortholog encodes a protein that binds FK506 and that the interaction between AtFKBP12 and AtFIP37 may involve the FK506 binding site of AtFKBP12. The interaction provides interesting new opportunities for controlling protein:protein interactions in vivo in plants.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adaptor Proteins, Signal Transducing*
  • Amino Acid Sequence
  • Arabidopsis / chemistry*
  • Cyclosporine / pharmacology
  • DNA, Plant / metabolism
  • Immunophilins / chemistry
  • Immunophilins / genetics
  • Immunophilins / metabolism*
  • Immunosuppressive Agents / pharmacology*
  • Molecular Sequence Data
  • Peptidylprolyl Isomerase / metabolism
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Tacrolimus / pharmacology*
  • Tacrolimus Binding Proteins


  • Adaptor Proteins, Signal Transducing
  • DNA, Plant
  • GLMN protein, human
  • Immunosuppressive Agents
  • Cyclosporine
  • Tacrolimus Binding Proteins
  • Immunophilins
  • Peptidylprolyl Isomerase
  • Tacrolimus

Associated data

  • GENBANK/AF084570