Isolation and chromosomal mapping of human glycogen synthase kinase-3 alpha and -3 beta encoding genes

Genome. 1998 Oct;41(5):720-7.


Glycogen synthase kinase-3 (GSK-3) is a serine-threonine kinase that exists as two isoforms, alpha and beta, encoded by separate genes. Phosphorylation targets include a variety of cytoplasmic and nuclear proteins. Recent studies found that neurofilaments, amyloid precursor protein, and tau proteins are substrates of GSK-3 and that aberrant phosphorylation of these proteins is implicated in pathologies of the nervous system. To analyse the organisation of these two genes, a YAC library was screened by polymerase chain reaction, using primers specific for human GSK-3 alpha and GSK-3 beta cDNA. Two clones, 220 and 285 kb in size, containing the complete GSK-3 alpha coding sequence, and two clones, 365 and 285 kb in size, containing the 5' coding sequence of GSK-3 beta, were isolated. By somatic cell hybrid panel DNA amplification and radiation hybrid mapping, GSK-3 alpha was found to be located at 19q13.2. On the other hand, by somatic cell hybrid panel DNA amplification and fluorescence in situ hybridisation using the 285-kb YAC clone, GSK-3 beta was mapped to 3q13.3.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Calcium-Calmodulin-Dependent Protein Kinases / genetics*
  • Chromosome Mapping*
  • Chromosomes, Artificial, Yeast / genetics
  • Chromosomes, Human, Pair 19 / genetics
  • Chromosomes, Human, Pair 3 / genetics
  • Cloning, Molecular
  • Cricetinae
  • DNA Primers / genetics
  • Glycogen Synthase Kinase 3
  • Glycogen Synthase Kinases
  • Humans
  • Hybrid Cells
  • In Situ Hybridization, Fluorescence
  • Isoenzymes / genetics*
  • Mice
  • Polymerase Chain Reaction


  • DNA Primers
  • Isoenzymes
  • Glycogen Synthase Kinases
  • Calcium-Calmodulin-Dependent Protein Kinases
  • Glycogen Synthase Kinase 3