Abstract
1. Whether basic fibroblast growth factor (bFGF) can stimulate proliferation and synthesis of chondroitin sulfate of chondrocytes in gel culture while maintaining the phenotype was studied. 2. At 3 weeks in culture, the cell number in 1.0 ng/ml (18.5+/-2.1 x 10(5)) and 10.0 ng/ml of the bFGF group (15.3+/-1.9 x 10(5)) was significantly larger than that in 0 ng/ml (12.3+/-2.1 x 10(5)), 0.1 ng/ ml (11.7+/-2.2 x 10(5)) and 100.0 ng/ml of the bFGF group (9.8+/-2.3 x 10(5)). 3. All doses of bFGF used in this study suppressed synthesis of chondroitin 6-sulfate. 4. Chondrocyte phenotype in gel culture was maintained for 4 weeks even with stimulation of bFGF.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cartilage, Articular / cytology
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Cartilage, Articular / drug effects
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Cartilage, Articular / metabolism
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Cartilage, Articular / ultrastructure
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Cell Count / drug effects
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Cell Division / drug effects
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Cells, Cultured
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Chondrocytes / cytology
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Chondrocytes / drug effects*
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Chondrocytes / metabolism
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Chondrocytes / ultrastructure
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Chondroitin Sulfates / antagonists & inhibitors
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Collagen
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Culture Media
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Dose-Response Relationship, Drug
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Extracellular Matrix / metabolism
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Fibroblast Growth Factor 2 / pharmacology*
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Gels
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Immunohistochemistry
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Phenotype
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Rabbits
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Tissue Embedding
Substances
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Culture Media
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Gels
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Fibroblast Growth Factor 2
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Chondroitin Sulfates
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Collagen