Estradiol protects against ischemic injury

J Cereb Blood Flow Metab. 1998 Nov;18(11):1253-8. doi: 10.1097/00004647-199811000-00012.


Clinical studies demonstrate that estrogen replacement therapy in postmenopausal women may enhance cognitive function and reduce neurodegeneration associated with Alzheimer's disease and stroke. This study assesses whether physiologic levels of estradiol prevent brain injury in an in vivo model of permanent focal ischemia. Sprague-Dawley rats were ovariectomized; they then were implanted, immediately or at the onset of ischemia, with capsules that produced physiologically low or physiologically high 17beta-estradiol levels in serum (10 or 60 pg/mL, respectively). One week after ovariectomy, ischemia was induced. Estradiol pretreatment significantly reduced overall infarct volume compared with oil-pretreated controls (mean+/-SD: oil = 241+/-88; low = 139+/-91; high = 132+/-88 mm3); this protective effect was regionally specific to the cortex, since no protection was observed in the striatum. Baseline and ischemic regional CBF did not differ between oil and estradiol pretreated rats, as measured by laser Doppler flowmetry. Acute estradiol treatment did not protect against ischemic injury. Our finding that estradiol pretreatment reduces injury demonstrates that physiologic levels of estradiol can protect against neurodegeneration.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Brain / blood supply
  • Brain Ischemia / physiopathology*
  • Brain Ischemia / prevention & control
  • Cerebral Infarction / physiopathology
  • Cerebral Infarction / prevention & control*
  • Cerebrovascular Circulation / drug effects
  • Cerebrovascular Circulation / physiology*
  • Drug Implants
  • Estradiol / administration & dosage
  • Estradiol / blood
  • Estradiol / therapeutic use*
  • Estrogen Replacement Therapy
  • Female
  • Humans
  • Ischemic Attack, Transient / physiopathology*
  • Ischemic Attack, Transient / prevention & control*
  • Laser-Doppler Flowmetry
  • Organ Specificity
  • Ovariectomy
  • Postmenopause
  • Rats
  • Rats, Sprague-Dawley


  • Drug Implants
  • Estradiol