This study was designed to investigate in the saliva the influence in female athletes of handball or volleyball training on concentrations of cortisol [C], dehydroepiandrosterone [DHEA], and on the [DHEA]:[C] ratio over 16 weeks of training. Data were compared to those of sedentary women. Saliva samples were collected upon waking after an overnight fast during the 1st week (W1) of the training programme and in the 16th week (W16). The training programme increased the resting concentrations of saliva [DHEA] in all the sportswomen. In contrast, a decrease of [DHEA] was noted in the sedentary group (W16 < W1; P < 0.05). In none of the women did the [C] at rest change significantly during the study. Between W1 and W16, the [DHEA]:[C] ratio increased by more than 30% in all the sportswomen. In addition, the athletes with the highest performance levels and greatest amount of training had the lowest [DHEA]:[C] ratio. Negative linear relationships between the amount of training and the [DHEA]:[C] ratio were found both at W1 (r = -0.53 P < 0.001), and W16 (r = -0.73 P < 0.001), suggesting that the latter could be used as an indicator of the training status of sportswomen.