Analysis of the length distribution of murein glycan strands in ftsZ and ftsI mutants of E. coli

FEMS Microbiol Lett. 1998 Nov 1;168(1):71-5. doi: 10.1111/j.1574-6968.1998.tb13257.x.

Abstract

The chain length distribution of murein glycan strands was analyzed in wild-type cells and in cells in which preseptal and/or septal murein synthesis was prevented in ftsZ84 and ftsI36 mutants of E. coli. This revealed a significant change in glycan chain lengths in newly synthesized murein associated with inactivation of the ftsZ gene product but not with inactivation of the ftsI gene product. This is the first reported abnormality in murein biosynthesis associated with mutation of an essential cell division gene.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacterial Proteins / genetics*
  • Carrier Proteins*
  • Cell Division
  • Chromatography, High Pressure Liquid
  • Cytoskeletal Proteins*
  • Escherichia coli / genetics*
  • Escherichia coli / growth & development
  • Escherichia coli / metabolism*
  • Hexosyltransferases / genetics*
  • Multienzyme Complexes / genetics*
  • Muramoylpentapeptide Carboxypeptidase*
  • Mutation
  • Penicillin-Binding Proteins
  • Peptidoglycan / biosynthesis*
  • Peptidoglycan / chemistry*
  • Peptidyl Transferases / genetics*

Substances

  • Bacterial Proteins
  • Carrier Proteins
  • Cytoskeletal Proteins
  • FtsZ protein, Bacteria
  • Multienzyme Complexes
  • Penicillin-Binding Proteins
  • Peptidoglycan
  • Peptidyl Transferases
  • Hexosyltransferases
  • Muramoylpentapeptide Carboxypeptidase