Underexpression of a novel gene, dia2, impairs the transition of Dictyostelium cells from growth to differentiation

Biochem Biophys Res Commun. 1998 Nov 9;252(1):278-83. doi: 10.1006/bbrc.1998.9461.

Abstract

In Dictyostelium discoideum Ax-2 cells, a specific point (PS-point) in the cell cycle from which they initiate differentiation in response to starvation has been specified. Using synchronized Ax-2 cells and the differential display method, a novel gene (differentiation-associated gene 2; dia2) was isolated as one of the genes expressed specifically during the shift of Ax-2 cells from growth to differentiation. The dia2 gene codes a lysine- and leucine-rich protein with a predicted molecular mass of 16.9 kDa. Northern blot analysis has shown that the dia2 mRNA, of 0.7 kb, accumulates in differentiating cells starved just before the PS-point, while there is no detectable expression in vegetatively growing cells. Antisense-mediated gene inactivation of dia2 greatly inhibited the progress of differentiation, presumably through the reduced expression of cAMP receptor 1 (car1). Thus, the DIA2 expression was suggested to have an essential role in the initiation of differentiation, closely relating to the cAMP signaling system.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Cycle / genetics*
  • Cell Differentiation
  • DNA Primers
  • Dictyostelium / cytology
  • Dictyostelium / genetics*
  • Dictyostelium / growth & development
  • Gene Deletion
  • Gene Expression Regulation, Developmental
  • Genes, Protozoan*
  • Molecular Sequence Data
  • Molecular Weight
  • Polymerase Chain Reaction
  • Promoter Regions, Genetic
  • Protozoan Proteins / biosynthesis
  • Protozoan Proteins / chemistry
  • Protozoan Proteins / genetics*

Substances

  • DNA Primers
  • Dia2 protein, Dictyostelium
  • Protozoan Proteins

Associated data

  • GENBANK/AB007027