Surfactant Protein A (SP-A) Gene Targeted Mice

Biochim Biophys Acta. 1998 Nov 19;1408(2-3):296-302. doi: 10.1016/s0925-4439(98)00075-1.

Abstract

Mice lacking surfactant protein A (SP-A) mRNA and protein in vivo were generated using gene targeting techniques. SP-A (-/-) mice have normal levels of SP-B, SP-C and SP-D mRNA and protein and survive and breed normally in vivarium conditions. Phospholipid composition, secretion and clearance, and incorporation of phospholipid precursors are normal in the SP-A (-/-) mice. Lungs of SP-A (-/-) mice have markedly decreased tubular myelin figures and clear Group B streptococci and Pseudomonas aeruginosa less efficiently than SP-A wild type mice. These studies of SP-A (-/-) mice demonstrate that SP-A has an important role in the innate immune system of the lung in vivo.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Animals
  • Bacteria / immunology
  • Gene Targeting
  • Immunity, Innate
  • Lung / immunology*
  • Lung / ultrastructure
  • Mice
  • Mice, Mutant Strains
  • Myelin Sheath / ultrastructure
  • Phagocytosis
  • Proteolipids / genetics
  • Proteolipids / immunology*
  • Pulmonary Surfactant-Associated Protein A
  • Pulmonary Surfactant-Associated Proteins
  • Pulmonary Surfactants / genetics
  • Pulmonary Surfactants / immunology*

Substances

  • Proteolipids
  • Pulmonary Surfactant-Associated Protein A
  • Pulmonary Surfactant-Associated Proteins
  • Pulmonary Surfactants