An autoregulatory cascade of EGF receptor signaling patterns the Drosophila egg

Cell. 1998 Oct 30;95(3):355-64. doi: 10.1016/s0092-8674(00)81767-5.

Abstract

Intercellular signaling through the EGF receptor (EGFR) patterns the Drosophila egg. The TGF alpha-like ligand Gurken signals from the oocyte to the receptor in the overlying somatic follicle cells. We show that in the dorsal follicle cells this initial paracrine signaling event triggers an autocrine amplification by two other EGFR ligands, Spitz and Vein. Spitz only becomes an effective ligand in the presence of the multitransmembrane domain protein Rhomboid. Consequent high-level EGFR activation leads to localized expression of the diffusible inhibitor Argos, which alters the profile of signaling. This sequential activation, amplification, and local inhibition of the EGFR forms an autoregulatory cascade that leads to the splitting of an initial single peak of signaling into two, thereby patterning the egg.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Autocrine Communication
  • Body Patterning*
  • Drosophila Proteins*
  • Drosophila melanogaster / genetics
  • Drosophila melanogaster / metabolism*
  • Egg Shell / growth & development
  • Epidermal Growth Factor*
  • ErbB Receptors / antagonists & inhibitors
  • ErbB Receptors / genetics
  • ErbB Receptors / metabolism*
  • Eye Proteins / genetics
  • Eye Proteins / metabolism
  • Female
  • Genes, Essential
  • Insect Proteins / genetics
  • Insect Proteins / metabolism
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism
  • Models, Biological
  • Mutation
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / metabolism
  • Neuregulins*
  • Oocytes / cytology
  • Oocytes / growth & development*
  • Oogenesis*
  • Ovarian Follicle / metabolism
  • Paracrine Communication
  • Phenotype
  • Signal Transduction*
  • Transforming Growth Factor alpha*
  • Transforming Growth Factors / genetics
  • Transforming Growth Factors / metabolism
  • ras Proteins / genetics

Substances

  • Drosophila Proteins
  • Eye Proteins
  • Insect Proteins
  • Membrane Proteins
  • Nerve Tissue Proteins
  • Neuregulins
  • Rho protein, Drosophila
  • Stet protein, Drosophila
  • Transforming Growth Factor alpha
  • grk protein, Drosophila
  • aos protein, Drosophila
  • spi protein, Drosophila
  • vn protein, Drosophila
  • Epidermal Growth Factor
  • Transforming Growth Factors
  • ErbB Receptors
  • ras Proteins