Changes in location and number of tartrate-resistant acid phosphatase (TRAP)-positive cells during the development of type II collagen-induced arthritis in DBA/1J mice

Exp Anim. 1998 Jul;47(3):211-4. doi: 10.1538/expanim.47.211.


The authors investigated changes in the location and number of osteoclasts and their precursors during the development of articular lesions in type II collagen-induced arthritis in mice using tartrate-resistant acid phosphatase (TRAP) staining. The limb joints were examined at 6 to 15 weeks after the second immunization. The number of TRAP-positive cells increased as the articular lesions progressed. TRAP-positive macrophage-like cells were found in the hyperplastic synovial tissue and bone marrow stroma in the early stage. In the advanced stage, in addition to many TRAP-positive osteoclasts on the bone surface, TRAP-positive macrophage-like cells were observed in the pannus apart from the bone surface in the pannus-joint junctions. The above mentioned TRAP-positive macrophage-like cells are considered to be osteoclast precursors.

MeSH terms

  • Acid Phosphatase / analysis*
  • Animals
  • Arthritis, Experimental / etiology
  • Arthritis, Experimental / pathology*
  • Cell Count
  • Collagen / immunology*
  • Histocytochemistry
  • Isoenzymes / analysis*
  • Mice
  • Mice, Inbred DBA
  • Osteoclasts / enzymology*
  • Osteoclasts / pathology
  • Tartrate-Resistant Acid Phosphatase


  • Isoenzymes
  • Collagen
  • Acid Phosphatase
  • Acp5 protein, mouse
  • Tartrate-Resistant Acid Phosphatase