A simple approach for discovering common nonelectrophoretic enzyme variability: a heat denaturation study in Drosophila melanogaster

Biochem Genet. 1978 Apr;16(3-4):299-305. doi: 10.1007/BF00484086.


A simple procedure is described to detect genetic heterogeneity within electrophoretic classes at a locus in Drosophila, based on electrophoresis and heat denaturation studies. Temperature-resistant (tr) and temperature-sensitive (ts) isoelectrophoretic alleles at the phosphoglucomutase locus (Pgm) are present at polymorphic frequencies in natural and in laboratory populations of Drosophila melanogaster.

MeSH terms

  • Alleles
  • Animals
  • Drosophila melanogaster / genetics*
  • Genes
  • Genetic Variation*
  • Hot Temperature
  • Phosphoglucomutase / genetics*
  • Protein Denaturation


  • Phosphoglucomutase