Typing of human Mycobacterium avium isolates in Italy by IS1245-based restriction fragment length polymorphism analysis

N Lari; M Cavallini; L Rindi; E Iona; L Fattorini; C Garzelli

doi:10.1128/JCM.36.12.3694-3697.1998

Typing of human Mycobacterium avium isolates in Italy by IS1245-based restriction fragment length polymorphism analysis

J Clin Microbiol. 1998 Dec;36(12):3694-7. doi: 10.1128/JCM.36.12.3694-3697.1998.

Authors

N Lari¹, M Cavallini, L Rindi, E Iona, L Fattorini, C Garzelli

Affiliation

¹ Dipartimento di Patologia Sperimentale, Biotecnologie Mediche, Infettivologia ed Epidemiologia, Università di Pisa, I-56127 Pisa, Italy.

Abstract

All but 2 of 63 Mycobacterium avium isolates from distinct geographic areas of Italy exhibited markedly polymorphic, multibanded IS1245 restriction fragment length polymorphism (RFLP) patterns; 2 isolates showed the low-number banding pattern typical of bird isolates. By computer analysis, 41 distinct IS1245 patterns and 10 clusters of essentially identical strains were detected; 40% of the 63 isolates showed genetic relatedness, suggesting the existence of a predominant AIDS-associated IS1245 RFLP pattern.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acquired Immunodeficiency Syndrome / microbiology
Bacterial Typing Techniques*
DNA Transposable Elements*
Humans
Mycobacterium avium / classification*
Polymorphism, Restriction Fragment Length*

Substances

DNA Transposable Elements