Distribution of neuronal intermediate filament proteins in the developing mouse olfactory system

J Neurosci Res. 1998 Nov 1;54(3):353-63. doi: 10.1002/(SICI)1097-4547(19981101)54:3<353::AID-JNR6>3.0.CO;2-6.


The distribution of neuronal intermediate filament proteins in the developing mouse olfactory bulb and olfactory epithelium was characterized by immunocytochemical approach. Antibodies against alpha-internexin, neurofilament triplet proteins (NFTPs; NF-L, NF-M, and NF-H) and peripherin were used to determine their expression at different developmental stages. Alpha-internexin and peripherin were first found to be co-localized in the olfactory neuroepithelium during early development. At the perinatal stage, expression patterns of alpha-internexin and peripherin are distinguishable by spatial and temporal manner: peripherin is predominantly expressed in the olfactory nerves; whereas alpha-internexin is expressed in both olfactory nerves and olfactory bulb. Our observation suggests that peripherin as well as alpha-internexin may play some roles in the process formation of olfactory nerves during development. In the developing olfactory periglomerulus, alpha-internexin was found around postnatal Day 3, whereas NFTPs were not observed until postnatal Day 7. Our data showed that the expression of alpha-internexin preceded those of the NFTPs in most neurons of the developing olfactory bulb. Some small neurons in the adult olfactory bulb were uniquely labeled with antibody to alpha-internexin. Our results suggest that alpha-internexin may play a functional role in the neuronal cytoarchitecture of developing olfactory system, and can be a neuronal marker for detecting postmitotic migrating neurons in the adult olfactory bulb.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carrier Proteins / metabolism
  • Dendrites / metabolism
  • Epithelium / metabolism
  • Fluorescent Antibody Technique, Indirect
  • Gene Expression Regulation, Developmental
  • Intermediate Filament Proteins / metabolism*
  • Membrane Glycoproteins*
  • Mice
  • Mice, Inbred ICR
  • Nerve Tissue Proteins / metabolism
  • Neurofilament Proteins / metabolism
  • Neurons / metabolism*
  • Olfactory Bulb / embryology
  • Olfactory Bulb / growth & development
  • Olfactory Bulb / metabolism*
  • Olfactory Nerve / embryology
  • Olfactory Nerve / growth & development
  • Olfactory Nerve / metabolism*
  • Peripherins
  • Vimentin / metabolism


  • Carrier Proteins
  • Intermediate Filament Proteins
  • Membrane Glycoproteins
  • Nerve Tissue Proteins
  • Neurofilament Proteins
  • Peripherins
  • Vimentin
  • alpha-internexin