In vitro cytotoxicity of recombinant human-TNF-alpha and actinomycin D on canine normal and tumor cells

J Vet Med Sci. 1998 Oct;60(10):1087-91. doi: 10.1292/jvms.60.1087.


The in vitro cytotoxicity of recombinant human tumor necrosis factor-alpha (rh-TNF-alpha) and actinomycin D (ACT-D) on canine normal and tumor cells was investigated. rh-TNF-alpha showed dose-dependent cytotoxic and cell-growth inhibitory effects on cultured canine kidney carcinoma cells (CKCa-1). rh-TNF-alpha alone produced little cytotoxic effect on canine normal cells. However, combined with ACT-D, it showed moderate cytotoxicity on normal canine cells from the kidney medulla, spleen, heart muscle and lung. When the effects on the spontaneous tumor cells were examined, the combination of rh-TNF-alpha and ACT-D produced substantial cytotoxic effect on 60% of the tumor cells. All mammary mixed tumors and perianal gland tumors tested were susceptible to this combination. The data indicated the combination of rh-TNF-alpha and ACT-D have in vitro cytotoxicity on certain canine tumor cells.

MeSH terms

  • Animals
  • Antibiotics, Antineoplastic / pharmacology*
  • Cell Division / drug effects
  • Cell Survival / drug effects
  • Cells, Cultured
  • Dactinomycin / administration & dosage
  • Dactinomycin / pharmacology*
  • Dog Diseases / pathology
  • Dogs
  • Dose-Response Relationship, Drug
  • Drug Synergism
  • Female
  • Humans
  • Kidney / cytology
  • Kidney / drug effects
  • Kidney Neoplasms / pathology
  • Kidney Neoplasms / veterinary
  • Male
  • Mammary Neoplasms, Animal / pathology
  • Recombinant Proteins / administration & dosage
  • Recombinant Proteins / pharmacology
  • Tumor Cells, Cultured
  • Tumor Necrosis Factor-alpha / administration & dosage
  • Tumor Necrosis Factor-alpha / pharmacology*


  • Antibiotics, Antineoplastic
  • Recombinant Proteins
  • Tumor Necrosis Factor-alpha
  • Dactinomycin