A simple and rapid method for preparing genomic DNA from gram-positive bacteria

Mol Biotechnol. 1998 Oct;10(2):191-3. doi: 10.1007/BF02760866.


Molecular epidemiologic and other studies may require preparation of genomic DNA from large numbers of bacteria in sufficiently pure form for restriction endonuclease digestion, cloning, RAPD-PCR, Southern hybridization, and so on. Staphylococcus and other Gram-positive bacteria have a rigid cell wall and can be difficult to lyse. Here, a simple and rapid method for the preparation of genomic DNA from multiple samples is reported. This method produces clean DNA for use in most molecular biology methods in < 90 min.

MeSH terms

  • Bacillus / genetics
  • DNA, Bacterial / genetics
  • DNA, Bacterial / isolation & purification*
  • Genome, Bacterial*
  • Gram-Positive Bacteria / genetics*
  • Reproducibility of Results
  • Staphylococcus / genetics


  • DNA, Bacterial