Wounding and chemicals induce expression of the Arabidopsis thaliana gene Thi2.1, encoding a fungal defense thionin, via the octadecanoid pathway

FEBS Lett. 1998 Oct 23;437(3):281-6. doi: 10.1016/s0014-5793(98)01251-4.


In seedlings of Arabidopsis thaliana the thionin gene Thi2.1 is inducible by methyl jasmonate, wounding, silver nitrate, coronatine, and sorbitol. We have used a biochemical and genetic approach to test the signal transduction of these different inducers. Both exogenously applied jasmonates and jasmonates produced endogenously upon stress induction, lead to GUS expression in a Thi2.1 promoter-uidA transgenic line. No GUS expression was observed in a coil mutant background which lacks jasmonate perception whereas methyl jasmonate and coronatine but not the other inducers were able to overcome the block in jasmonic acid production in a fad3-2 fad7-2 fad8 mutant background. Our results show conclusively that all these inducers regulate Thi2-1 gene expression via the octadecanoid pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antifungal Agents*
  • Antimicrobial Cationic Peptides
  • Arabidopsis / drug effects
  • Arabidopsis / genetics*
  • Arabidopsis / metabolism
  • Arabidopsis Proteins*
  • Cyclopentanes / antagonists & inhibitors
  • Cyclopentanes / metabolism
  • Gene Expression Regulation, Plant* / drug effects
  • Mutation
  • Oxylipins
  • Plant Proteins / biosynthesis*
  • Plant Proteins / genetics*
  • Plant Proteins / metabolism
  • Silver Nitrate / pharmacology
  • Sorbitol / pharmacology
  • Stearic Acids / metabolism*


  • Antifungal Agents
  • Antimicrobial Cationic Peptides
  • Arabidopsis Proteins
  • Cyclopentanes
  • Oxylipins
  • Plant Proteins
  • Stearic Acids
  • THI2.1 protein, Arabidopsis
  • Sorbitol
  • jasmonic acid
  • Silver Nitrate