The SH2 Domain Containing Inositol 5-phosphatase SHIP2 Displays Phosphatidylinositol 3,4,5-trisphosphate and Inositol 1,3,4,5-tetrakisphosphate 5-phosphatase Activity

FEBS Lett. 1998 Oct 23;437(3):301-3. doi: 10.1016/s0014-5793(98)01255-1.

Abstract

Distinct forms of inositol and phosphatidylinositol polyphosphate 5-phosphatases selectively remove the phosphate from the 5-position of the inositol ring from both soluble and lipid substrates. SHIP1 is the 145-kDa SH2 domain-containing inositol 5-phosphatase expressed in haematopoietic cells. SHIP2 is a related but distinct gene product. We report here that SHIP2 can be expressed in an active form both in Escherichia coli and in COS-7 cells. A truncated 103-kDa recombinant protein could be purified from bacteria that display both inositol 1,3,4,5-tetrakisphosphate (InsP4) and phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) phosphatase activities. COS-7 cell lysates transfected with SHIP2 had increased PtdIns(3,4,5)P3 phosphatase activity as compared to the vector alone.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • COS Cells
  • Enzyme Activation / genetics
  • Escherichia coli / genetics
  • Gene Expression
  • Humans
  • Inositol Phosphates / metabolism*
  • Phosphatidylinositol Phosphates / metabolism*
  • Phosphatidylinositol-3,4,5-Trisphosphate 5-Phosphatases
  • Phosphoric Monoester Hydrolases / biosynthesis
  • Phosphoric Monoester Hydrolases / genetics
  • Phosphoric Monoester Hydrolases / metabolism*
  • Recombinant Proteins / biosynthesis
  • src Homology Domains*

Substances

  • Inositol Phosphates
  • Phosphatidylinositol Phosphates
  • Recombinant Proteins
  • phosphatidylinositol 3,4,5-triphosphate
  • inositol-1,3,4,5-tetrakisphosphate
  • phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase
  • Phosphoric Monoester Hydrolases
  • INPPL1 protein, human
  • Phosphatidylinositol-3,4,5-Trisphosphate 5-Phosphatases